The monocyte activation test (MAT) is a test based on the human reaction to pyrogens that cause fever and is widely used as an alternative to the rabbit pyrogen test. Its basic principle is that Toll-like receptors mediate the stimulation of blood monocytes to produce pro-inflammatory cytokines in response to various pyrogens. The level of released IL-1ß was determined by classical IL-1ß ELISA. The method can be used to detect a wide range of pyrogens, including Gram-positive and Gram-negative organisms, yeast, viruses, and parasites, etc. MAT can also provide new solutions for products that have proven problematic in other in vitro endotoxin tests, such as drugs that cause immunological responses, drugs that affect thermoregulation (such as antipyretics and steroids), certain blood-derived products, detergents, and products that interfere with clotting, etc.
Due to the complexity of the experiment and the demands of the market, we offer MAT proficiency tests to provide quality assurance to laboratories performing MAT. Based on our advanced platform and professional technicians, we are capable of offering high-quality MAT audits.
Figure 1. The biological principle of the "Monocyte Activation Test" as defined by the monograph 2.6.30 in the European Pharmacopoeia (Hasiwa, N.; et al. 2013)
As experts in endotoxin research, we have accumulated a lot of experience in this field. Our MAT proficiency test is in accordance with European Pharmacopoeia (EP) and provides services including validation of working procedures, methods, and reports, as well as training services for laboratories in need.
Our experts will compare your results with those from other laboratories in a confidential way to test and verify the accuracy, allowing customers to improve their MAT methods based on the evaluation results. Through our services, customers can enrich their MAT expertise and ensure full MAT-compliance.
As a leading expert in endotoxin research, Creative BioMart has the solid expertise and scientific background to provide strong technical support to researchers around the world. In order to provide customers with the accuracy of the MAT experiment, we provide a comprehensive MAT proficiency test, and all procedures will be conducted in a confidential manner. If you are interested in our services, please contact us for more details.
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