Endotoxin Removal Services in Recombinant Protein

Recombinant protein is a protein obtained by using genetic recombination technology. First, a recombinant vector is introduced, a gene fragment that can be translated into a target protein is ligated to the recombinant vector, and then transferred to a host cell that can express the target protein to express a specific recombinant protein molecule. Currently, the production of recombinant proteins mainly includes four major systems: prokaryotic protein expression, mammalian cell protein expression, yeast protein expression, and insect cell protein expression. As a biopharmaceutical product, the resulting recombinant protein plays an important role in medicine. After purification of the recombinant vector plasmid, endotoxin may adversely affect some downstream applications. If the endotoxin level is high enough, it will affect the transfection process of the cell's DNA, resulting in reduced transfection efficiency.

Why should we remove endotoxin from recombinant protein?

Endotoxin is widely found in the cell walls of Gram-negative bacteria, such as E. coli. In the process of expressing recombinant protein, E. coli produces macroendotoxin. Endotoxin, as a heat source, leads to inflammatory and febrile reactions in the organism, so when the recombinant protein is used in some animal experiments, endotoxin needs to be removed in order to avoid causing adverse reactions in the animals and thus affecting the experimental results. Endotoxin may also be used for gene therapy of polypeptides and antibodies produced by recombinant proteins Applications have an adverse effect because they can cause side effects such as fever in animals and humans and endotoxemia, leading to misunderstandings of research results. In summary, it is of great significance to detect and remove endotoxin in recombinant proteins.

The general process of endotoxin removal

Endotoxin control and removal is based on the principle of endotoxin-binding peptide purification resin, which needs to be controlled from the beginning of protein preparation experiments. Practical utensils, instruments, water, reagents, and pipettes are used for endotoxin removal. At the same time, the experiments were performed in a clean room, ensuring that all levels of low endotoxin tests were achieved.

The general process of endotoxin removal.Fig1. The general process of endotoxin removal.

Our endotoxin removal services

Creative BioMart offers a variety of endotoxin removal services covering all existing endotoxin removal methods to meet the specific needs of different customers.

Table.1 Comparison of our endotoxin removal methods

Method Application Advantages Disadvantages
Affinity chromatography Specific adsorption
  • High efficiency
  • High selectivity
  • High cost
Ultrafiltration A small molecular weight of the target product
  • Simple operation
  • Large processing capacity
  • Retaining or adsorbing active ingredients in the sample

Endotoxin removal services in recombinant protein

Creative BioMart offers a corresponding endotoxin removal service. You can purchase the corresponding endotoxin removal kit and related accessory products according to the needs of your own samples. We guarantee that all instruments, water, reagents, and consumables used in the experiment are free of endotoxins, and the experiment is conducted in a clean room to ensure that low levels of endotoxins are returned to the sample. In addition, we can also provide you with related services including:

Our solutions

Project name Endotoxin removal services in recombinant protein
Testing cycle 5-7 days.
Service including Creative BioMart can screen for the source of endotoxin contamination in the production process and propose several solutions to remove endotoxin to reduce the endotoxin content in the finished product.
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