Endotoxin Testing in Cerebrospinal Fluid

Bacterial meningitis is a dangerous disease, and early diagnosis is of great significance. Endotoxin in cerebrospinal fluid is relatively stable and is not cleared by endothelial cells. Endotoxin in the same shirt circulation cannot pass the blood-brain barrier. Some data indicate that the testing rate of cerebrospinal fluid endotoxin is as high as 90% for bacterial meningitis.

Cerebrospinal systems.Figure 1. Cerebrospinal systems.


CSF comes from plasma, which is basically similar to plasma, except that CSF has almost no protein compared to plasma, and the electrolyte levels are different. Due to its production method, CSF has higher chloride content than plasma and comparable sodium content. CSF contains about 0.3% of plasma protein, or about 15 to 40 mg/dL, depending on the sampling site. Generally, globulin and albumin have lower concentrations in ventricular CSF compared to lumbar spine or pleural fluid. This continuous flow of blood into the venous system dilutes the concentration of larger lipid-insoluble molecules that penetrate the brain and CSF. CSF usually does not contain red blood cells and contains less than 5 white blood cells per mm³. Any cell with white blood cell counts higher than this number will constitute endocytosis. CSF contains nucleic acids, especially cell-free DNA.

Figure 2. Distribution of CSF.


Creative BioMart uses Limulus Amebocyte Lysate test for urine endotoxin testing. The primary application for LAL is the testing of blood or cerebrospinal fluid. There are three basic methodologies: gel-clot, turbidimetric, and chromogenic. The primary application for LAL is the testing of parenteral pharmaceuticals and medical devices that contact blood or cerebrospinal fluid. In the United States, the FDA has published a guideline for validation of the LAL test as an endotoxin test for such products. Limulus amebocyte lysate (LAL) is an aqueous extract of blood cells (amoebocytes) from the Atlantic horseshoe crab, Limulus polyphemus. LAL reacts with bacterial endotoxin lipopolysaccharide (LPS), which is a membrane component of gram-negative bacteria. This reaction is the basis of the LAL test, which is widely used for the testing and quantification of bacterial endotoxins. In Asia, a similar Tachypleus amebocyte lysate (TAL) test based on the local horseshoe crabs Tachypleus gigas or Tachypleus tridentatus is occasionally used instead. The recombinant factor C (rFC) assay is a replacement of LAL/TAL based on a similar reaction.

Our Services for endotoxin testing in cerebrospinal fluid

Creative BioMart offers a corresponding endotoxin testing service. You can purchase the corresponding endotoxin removal kit and related accessory products according to the needs of your own samples. We guarantee that all instruments, water, reagents, and consumables used in the experiment are free of endotoxins, and the experiment is conducted in a clean room to ensure that low levels of endotoxins are returned to the sample. In addition, we can also provide you with related services including:

Our solutions

Project name Endotoxin testing in cerebrospinal fluid
Testing purpose Endotoxin in cerebrospinal fluid is relatively stable and cannot be cleared by endothelial cells. For bacterial meningitis, the detection rate of cerebrospinal fluid endotoxin is as high as 90%. The detection of endotoxin in cerebrospinal fluid is of great significance.
Testing cycle 3-5 days.
Service including We provide you with raw data and test reports.
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  1. Mouliere F; et al. Testing of cell-free DNA fragmentation and copy number alterations in cerebrospinal fluid from glioma patients. EMBO Mol Med. 2018,10 (12): e9323.
  2. Iwanaga, S. Biochemical principle of Limulus test for detecting bacterial endotoxins. Proceedings of the Japan Academy. Series B, Physical and biological sciences. 2007, 83 (4): 110–9.

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