Antibodies refer to proteins with protective effects produced by the body due to the stimulation of antigens. It (immunoglobulin is not just an antibody) is a large Y-shaped protein secreted by plasma cells (effector B cells) and used by the immune system to identify and neutralize foreign substances such as bacteria and viruses. The surface of cell membranes of vertebrate blood and other B cells. Antibodies recognize a unique feature of a particular foreign object, which is called an antigen. Endotoxin is widely present in environmental media such as air, soil and water. After endotoxin-contaminated water enters the human body, small doses of endotoxin (1-5ng/kg body weight) can cause the body temperature to rise, produce endotoxin shock, and disseminate intravascular coagulation, which seriously threatens the health of the population. Endotoxin is not easy to be destroyed by heating and chlorination disinfection. Therefore, it is very important to monitor endotoxin in antibodies in real time.
Figure 1. Antibody.
Creative BioMart provides a full range of services for endotoxin testing. Our endotoxin testing services cover a wide range of methods and are available for a wide range of samples to provide comprehensive and efficient help for your experiments. Creative BioMart' antibody endotoxin testing service mainly uses chromogenic LAL analysis and Gel Clot LAL Assay.
Chromogenic LAL analysis uses a chromogenic substrate to detect the presence of endotoxin in solution. The dynamic color development method does not monitor the change in turbidity of the solution, but monitors the color change of the solution. The more endotoxins in the test solution, the faster the solution turns yellow. The color rendering LAL analysis service aims to detect endotoxins through a discoloration reaction caused by the interaction between LAL and endotoxin. The mechanism of this reaction is a complex enzyme-linked reaction. In the presence of LPS, factor C in LAL/TAL is activated, thereby activating factor B. Factor B activates thrombin to thrombin. Finally, thrombin cuts the chromogenic substrate into a yellow product. We measure endotoxin content by color depth.
The Gel Clot LAL Test Service is designed to detect endotoxins through the formation of gels through the interaction of LAL and endotoxins. The mechanism of this reaction is a complex enzyme-linked reaction, as shown in the figure below. In the presence of LPS, factor C in LAL/TAL is activated, thereby activating factor B. Factor B activates procoagulase to thrombin. Finally, lectin cleaves lectins into lectins, which bind together to form a clot. And we determine the presence or absence of endotoxin by forming a gel.
Creative BioMart offers a corresponding endotoxin testing service. You can purchase the corresponding endotoxin removal kit and related accessory products according to the needs of your own samples. We guarantee that all instruments, water, reagents, and consumables used in the experiment are free of endotoxins, and the experiment is conducted in a clean room to ensure that low levels of endotoxins are returned to the sample. In addition, we can also provide you with related services including:
|Project name||Endotoxin testing in antibody|
|Testing purpose||Endotoxins are widespread in the air, soil and water. After the water contaminated with endotoxin enters the human body, a small dose of endotoxin will cause the body temperature to rise, produce endotoxin shock and spread intravascular coagulation, thus seriously threatening people's health. Therefore, real-time monitoring of endotoxin in antibodies is very important.|
|Testing cycle||3-5 days.|
|Service including||We provide you with raw data and test reports.|