Endotoxins, also known as lipopolysaccharides (LPS), are major components of the outer membrane of Gram-negative bacteria. In addition to serving as a barrier to the environment and various toxic substances such as antibiotics, the outer membrane can also act as a nutrient transporter. LPS constitutes about 3.6% of the entire bacterial cell, covering almost three-quarters of the surface area of the outer membrane of E. coli. Typically, the term LPS refers to the purified form, while endotoxin refers to LPS attached on the bacterial membrane. Released from surfaces during bacterial growth, cell division, and lysis, LPS are toxic inflammatory factors ubiquitous in the environment that affect many cellular and humoral-mediated systems. Studies have found that endotoxins can also augment the toxicity of some chemicals, such as microcystins. Currently, endotoxins have been considered to be one of the main causes of pyrogenic reactions during biotherapeutics administration.
Due to the influence of LSP, there are more and more studies on it. The identification and characterization of intact LPSs are challenging areas of research, and there is an even greater need for sensitive, reproducible, and reliable LPS extraction methods to support them.
Figure 1. Structural details of lipopolysaccharide from a Gram-negative bacterium (Mazgaeen, L.; Gurung, P. 2020)
In order to accelerate the process of endotoxin research, Creative BioMart has introduced a large number of professional technical personnel to help various scholars and industrial companies to extract LPS from specific bacteria. To meet different research needs, we offer two LPS extraction schemes:
Less than 3 weeks
Providing quality testing and protein contamination testing (＜3% protein)
The obtained samples can be used for vaccine development
A gentle extraction method
Fast, ultra-pure production of LPS
No toxic residues
Quality testing can reach ＜1% protein contamination and ＜3% amino acid contamination
The obtained samples can be used for therapeutic development
As a leading expert in endotoxin research, Creative BioMart has the solid expertise and scientific background to provide strong technical support to researchers around the world. To speed up your research process, we offer efficient and reliable LPS extraction services that can help extract LPS from any kind of Gram-negative bacteria. If you are interested in our services, please contact us for more details.
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