Bacterial endotoxin in serum is closely related to many diseases, and endotoxemia is mostly related to the deterioration of the disease, and it decreases with the remission of the disease. Therefore, bacterial endotoxin can be used as an important reference index for measuring the condition and judging the prognosis. Endotoxemia can occur when a large number of Gram-negative pathogens die in the lesion or blood stream, and a large amount of endotoxin is released into the blood. A large number of endotoxins affect the body's macrophages, neutrophils, endothelial cells, platelets, as well as the complement system and the coagulation system, etc., which will produce interleukins 1, 6, 8 and tumor necrosis factor α, histamine, 5 Bioactive substances such as serotonin, prostaglandin, kinin. These substances act on small blood vessels and cause functional disturbances that lead to microcirculation disorders. Clinical manifestations include microcirculation failure, hypotension, hypoxia, acidosis, etc., which leads to shock. This pathological reaction is called endotoxin shock.
In addition to the above, it is helpful for departments such as infectious diseases, oncology, neurology (surgery), and heart medicine.
Creative BioMart's serum endotoxin test mainly uses Turbidimetric LAL Assay method, which is aims to detect endotoxin by changing the absorbance caused by the formation of a gel by LAL and endotoxin interactions. The mechanism of this reaction is a complex enzyme-linked reaction, as shown in the figure below. In the presence of LPS, factor C in LAL/TAL is activated, which activates factor B. Factor B activates the proclotting enzyme to clotting enzyme. Finally, the clotting enzyme cleaves the coagulogen into coagulin, which binds together to form a clot. The process of gel formation causes the solution to become cloudy and cause changes in absorbance, and we use this to determine the presence or absence of endotoxin.
Fig1. Mechanism of Turbidimetric LAL Assay
Creative BioMart offers a corresponding endotoxin testing service. You can purchase the corresponding endotoxin removal kit and related accessory products according to the needs of your own samples. We guarantee that all instruments, water, reagents, and consumables used in the experiment are free of endotoxins, and the experiment is conducted in a clean room to ensure that low levels of endotoxins are returned to the sample. In addition, we can also provide you with related services including:
|Project name||Endotoxin testing in serum|
|Testing purpose||Bacterial endotoxin in the serum is closely related to many diseases, and endotoxemia is mainly related to the deterioration of the disease, and decreases with the remission of the disease. Therefore, bacterial endotoxin can be used as an important reference index for measuring the condition and prognosis.|
|Testing cycle||3-5 days.|
|Service including||We provide you with raw data and test reports.|