The chemical nature of endotoxin is lipopolysaccharide (LPS), which consists of three parts, namely lipid A, core oligosaccharide and O-specific polysaccharide, which is a part of the structure of the outer membrane of the cell wall of Gram-negative bacteria. Most biotech drugs are prepared from the expression of Gram-negative bacteria. LPS content is usually high, and environmental or operational factors can easily lead to product contamination by LPS. LPS has a very strong heat source. A very small amount (ng level) can cause high fever, diarrhea, vasodilation, and even fainting or death. Therefore, the removal of LPS has been a hot topic in biotechnology pharmaceutical research. In recent years, research on the removal of LPS has made great progress, and the proportion of biotechnology products in the pharmaceutical industry has become larger and larger. The research and development of LPS removal processes and the expansion of industrialization scale are still the focus of future research. Over the years, we have successfully developed endotoxin removal programs to meet stringent endotoxin requirements. We are confident that you can make your endotoxin removal project a success.
Fig1. Endotoxin removal services.
Endotoxin removal is one of the most difficult tasks downstream in protein purification. At Creative BioMart, our experienced scientists will develop a customized protocol for your target protein after estimating endotoxin removal efficiency and sample recovery. We will use a combination of the following methods to ensure maximum efficiency.
The high selectivity of affinity chromatography eliminates the need for multiple purification steps and reduces production costs. At the same time, this method can remove endotoxin with high specificity and efficiency, resulting in excellent target recovery.
The advantages of this method are fast separation, wide selection of AEC media and disinfection of sodium hydroxide (NaOH), and no solvent is required.
With many years of research experience in the field of recombinant protein expression, Creative BioMart understands that the removal of endotoxin from biological samples is extremely important for downstream applications. We will be your reliable partner to effectively reduce endotoxin levels below 0.01 EU/µg and meet the stringent requirements of downstream animal and cell experiments. Contact us for project quotations and more details.
Fig2. The general process of endotoxin removal.
Creative BioMart offers a corresponding endotoxin removal service. You can purchase the corresponding endotoxin removal kit and related accessory products according to the needs of your own samples. We guarantee that all instruments, water, reagents, and consumables used in the experiment are free of endotoxins, and the experiment is conducted in a clean room to ensure that low levels of endotoxins are returned to the sample. In addition, we can also provide you with related services including:
|Project name||Endotoxin removal services in biologics|
|Removal cycle||5-7 days.|
|Removal purpose||Endotoxin content is usually very high, and environmental or operational factors can easily lead to LPS contamination of products. LPS has a very strong heat source. Very small amounts (ng content) can cause high fever, diarrhea, vasodilation, and even fainting or death. Therefore, the removal of LPS has always been an important step in biotechnology drug research.|
|Service including||Creative BioMart can screen for the source of endotoxin contamination in the production process and propose several solutions to remove endotoxin to reduce the endotoxin content in the finished product.|