Vaccine is a biological agent that provides active acquired immunity against a specific infectious disease. Vaccines often contain substances similar to pathogenic microorganisms and are usually made from weakened or killed microorganisms, one of their toxins or surface proteins. The substance stimulates the body's immune system, recognizes it as a threat, destroys it, and further identifies and destroys any microorganisms it may encounter in the future. Vaccines can be prophylactic (preventing or mitigating the effects of future infections by natural or "wild" pathogens) or therapeutic (eg, anti-cancer vaccines being studied). However, during the preparation of the vaccine, a large amount of Endotoxin of E. coli remains. Bacterial endotoxin is a lipopolysaccharide (LPS) on the outer membrane of the cell wall of Gram-negative bacteria. Normally, a 10% wet bacteria concentration can produce tens of thousands of EU / ml LPS, which is beyond the human tolerance range. LPS is highly pyrogenic, and small amounts can cause fever, impaired blood circulation, and even death from shock. Moreover, the presence of endotoxin seriously affects the transfection efficiency of cell. Bacterial endotoxin must be removed before vaccination, or serious consequences may result.
Endotoxin removal is one of the most difficult tasks downstream in protein purification. At Creative BioMart, our experienced scientists will develop a customized protocol for your target protein after estimating endotoxin removal efficiency and sample recovery. We will use a combination of the following methods to ensure maximum efficiency.
The hydrophobic column interacts with nonpolar protein surfaces through van der Waals forces for endotoxin removal.
It uses composite polyacrylamide as the column, which is highly porous. Ultrafiltration results in relatively good endotoxin clearance from product solutions when the products are of low molecular weight.
The high selectivity of affinity chromatography eliminates the need for multiple purification steps and reduces production costs. At the same time, this method can remove endotoxin with high specificity and efficiency, resulting in excellent target recovery.
Bacterial endotoxins are released during the growth and death of bacteria. After the cells rupture, a large amount of LPS is released into the solution. Endotoxin usually has a capsule-like structure, and its relative molecular weight, charge and hydrophobicity are similar to vaccine, which makes it very difficult to purify vaccines. In addition, the presence of endotoxin seriously affects the transfection efficiency of cells. Therefore, bacterial endotoxin must be removed before vaccination, or serious consequences may result.
Creative BioMart offers a corresponding endotoxin removal service. You can purchase the corresponding endotoxin removal kit and related accessory products according to the needs of your own samples. We guarantee that all instruments, water, reagents, and consumables used in the experiment are free of endotoxins, and the experiment is conducted in a clean room to ensure that low levels of endotoxins are returned to the sample. In addition, we can also provide you with related services including:
|Project name||Endotoxin removal services in vaccine|
|Removal cycle||5-7 days.|
|Removal purpose||During the preparation of the vaccine, a large amount of endotoxin remained. Bacterial endotoxin is lipopolysaccharide (LPS) on the outer membrane of the cell wall of Gram-negative bacteria. LPS is highly pyrogenic, a small amount can cause fever, blood circulation disorder, and even death due to shock. In addition, the presence of endotoxin seriously affects the transfection efficiency of cells. The bacterial endotoxin must be removed before vaccination, otherwise it may cause serious consequences.|
|Service including||Creative BioMart can screen for the source of endotoxin contamination in the production process and propose several solutions to remove endotoxin to reduce the endotoxin content in the finished product.|