Creative BioMart is one of the recognized experts in endotoxin testing. We have many years of experience in endotoxin testing and advanced production systems and equipment to provide fast and satisfactory endotoxin testing services according to customer needs.
Bacterial endotoxin is a lipopolysaccharide complex of Gram-negative bacteria and part of the cyanobacteria cell wall, which is mainly released by the death of the bacteria. Endotoxin contamination of water in contact with blood such as dialysis water and pharmaceutical water is also concerned and strictly controlled. In recent years, the health risks caused by endotoxins in drinking water and the water environment have begun to receive attention. When microbial proliferation or cyanobacteria outbreaks occur in the water environment, endotoxins released by bacterial death can cause potential health risks through exposure to blood, breathing, and gastrointestinal tract. Although bacterial endotoxins have not been included in drinking water and reclaimed water quality standards at home and abroad, in recent years, research on endotoxin pollution in surface water, groundwater, drinking water, reclaimed water, and effluent from sewage treatment plants has increased year by year. In contrast, more attention should be paid to the testing of endotoxins in drinking water.
Figure 1. Typical structure of endotoxin.
Gas chromatography-mass spectrometry (GC-MS) was used to characterize the endotoxin content of the 3-hydroxy fatty acid of the endotoxin molecule, but the test results showed that there was no uniform correlation between the content of 3-hydroxy fatty acid and the biological activity of endotoxin. Sex. Because 3-hydroxy fatty acid is a typical structure of lipid A in endotoxin molecules and is not an active group, GC-MS testing of 3-hydroxy fatty acids in endotoxin molecules cannot reflect the biological activity of endotoxins, thus limiting the method's Further application.
LAL test is a kind of arthropod. Amebocytes in the blood contain factor C, factor B and proclottin-gEnzyme, which can be activated by endotoxin. The activated coagulase can make the coagulation in the deformed cells coagulagen is coagulated to form coagulated proteins, which can be used for qualitative, semi-quantitative and quantitative determination of endotoxin. The reaction has high sensitivity (pg/ml) and specificity. It can be divided into gel method, turbidity method and color matrix method. The sensitivity of the LAL test is much higher than that of the rabbit method, and the operation is simple, the cost is low, the degree of standardization is high, and the repeatability is good. Since 1980, the 20th edition of the United States Pharmacopoeia included radon trials, pharmacopoeia and other countries have successively included this method.
Creative BioMart offers a corresponding endotoxin detection service. You can purchase the corresponding endotoxin removal kit and related accessory products according to the needs of your own samples. We guarantee that all instruments, water, reagents, and consumables used in the experiment are free of endotoxins, and the experiment is conducted in a clean room to ensure that low levels of endotoxins are returned to the sample. In addition, we can also provide you with related services including:
|Project name||Endotoxin testing in running water|
|Testing purpose||Although neither drinking water nor reclaimed water quality standards contain bacterial endotoxins, in recent years, studies on endotoxin pollution in surface water, groundwater, drinking water, reclaimed water and wastewater from wastewater treatment plants have increased year by year. More attention should be paid to the detection of endotoxin in drinking water.|
|Testing cycle||3-5 days.|
|Service including||We provide you with raw data and test reports.|