Among infectious diseases, Gram-negative bacteria (GNB) infection has the highest clinical mortality rate for sepsis, endotoxemia, and shock. During GNB infection, bacteria and lipopolysaccharide enter the blood circulation, activate various inflammatory cells to release inflammatory mediators, cause inflammatory reactions, and in severe cases can lead to multiple organ failure. LPS is the main component of the GNB outer membrane. It consists of lipid A, core oligosaccharides and O-specific polysaccharide side chains. LPS plays a major role in maintaining GNB's outer membrane permeability and migration and its pathogenic process. LPS can also directly or indirectly induce the apoptosis of immune cells and suppress the body's immune function, thereby exacerbating the occurrence of sepsis. Therefore, the testing of lipopolysaccharide has important medical significance. Recent research shows that DNA and RNA not only play a role in storing and transmitting genetic information, but can also interact with other types of molecules through their own unique structures. Based on this, an artificially designed random oligonucleotide library can be established. This is also very important for detecting endotoxin in nucleic acids.
Creative BioMart provides a full range of services for endotoxin testing. Our endotoxin testing services cover a wide range of methods and are available for a wide range of samples to provide comprehensive and efficient help for your experiments. Creative BioMart' nucleic acid endotoxin testing service mainly uses chromogenic LAL analysis and Gel Clot LAL Assay.
Chromogenic LAL analysis uses a chromogenic substrate to detect the presence of endotoxin in solution. The dynamic color development method does not monitor the change in turbidity of the solution, but monitors the color change of the solution. The more endotoxins in the test solution, the faster the solution turns yellow. The color rendering LAL analysis service aims to detect endotoxins through a discoloration reaction caused by the interaction between LAL and endotoxin. The mechanism of this reaction is a complex enzyme-linked reaction. In the presence of LPS, factor C in LAL/TAL is activated, thereby activating factor B. Factor B activates thrombin to thrombin. Finally, thrombin cuts the chromogenic substrate into a yellow product. We measure endotoxin content by color depth.
The Gel Clot LAL Test Service is designed to detect endotoxins through the formation of gels through the interaction of LAL and endotoxins. The mechanism of this reaction is a complex enzyme-linked reaction, as shown in the figure below. In the presence of LPS, factor C in LAL / TAL is activated, thereby activating factor B. Factor B activates procoagulase to thrombin. Finally, lectin cleaves lectins into lectins, which bind together to form a clot. And we determine the presence or absence of endotoxin by forming a gel.
Creative BioMart offers a corresponding endotoxin testing service. You can purchase the corresponding endotoxin removal kit and related accessory products according to the needs of your own samples. We guarantee that all instruments, water, reagents, and consumables used in the experiment are free of endotoxins, and the experiment is conducted in a clean room to ensure that low levels of endotoxins are returned to the sample. In addition, we can also provide you with related services including:
| Project name | Endotoxin testing in nucleic acid |
| Testing purpose | Gram-negative bacteria (GNB) infection has the highest clinical mortality rate for sepsis, endotoxemia, and shock. During GNB infection, bacteria and lipopolysaccharide enter the blood circulation, activate various inflammatory cells to release inflammatory mediators, cause inflammatory reactions, and in severe cases can lead to multiple organ failure. |
| Testing cycle | 3-5 days. |
| Service including | We provide you with raw data and test reports. |
| Price | Inquiry |
