Creative BioMart is one of the recognized experts in endotoxin testing. We have many years of experience in endotoxin testing and advanced production systems and equipment to provide fast and satisfactory endotoxin testing services according to customer needs.
There are many situations in clinical application that require endotoxin testing, otherwise it will cause serious consequences. For example, in the sterilization process of the hemodialysis equipment system, dialysis water, dialysis concentration and dialysate that exceed the endotoxin level will bring endotoxin into the blood of uremic patients, causing endotoxemia in uremic patients. Endotoxin acts on phagocytes, neutrophils, endothelial cells, platelets, the complement system and the coagulation system, and induces tumor necrosis factor-α (TNF-a) and leukocytes. Bioactive substances such as interleukins (IL-1, see Figure 1), IL-6 and IL-8, histamine and kallikrein can cause insufficient capillary perfusion of tissues and organs, resulting in hypoxia and acidosis. High concentrations of endotoxin can also activate alternative complement pathways, trigger high fever, low blood pressure and activate the coagulation system, leading to disseminated intravascular coagulation. In severe cases, it may cause endotoxin shock or even death, characterized by microcirculation failure and hypotension. After the cause is identified, the infected person should be given anti-infective treatment.
Gas chromatography-mass spectrometry (GC-MS) was used to characterize the endotoxin content of the 3-hydroxy fatty acid of the endotoxin molecule, but the test results showed that there was no uniform correlation between the content of 3-hydroxy fatty acid and the biological activity of endotoxin. Sex. Because 3-hydroxy fatty acid is a typical structure of lipid A in endotoxin molecules and is not an active group, GC-MS testing of 3-hydroxy fatty acids in endotoxin molecules cannot reflect the biological activity of endotoxins, thus limiting the method's Further application.
LAL test is a kind of arthropod. Amebocytes in the blood contain factor C, factor B and proclottin-gEnzyme, which can be activated by endotoxin. The activated coagulase can make the coagulation in the deformed cells coagulagen is coagulated to form coagulated proteins, which can be used for qualitative, semi-quantitative and quantitative determination of endotoxin. The reaction has high sensitivity (pg/ml) and specificity. It can be divided into gel method, turbidity method and color matrix method. The sensitivity of the LAL test is much higher than that of the rabbit method, and the operation is simple, the cost is low, the degree of standardization is high, and the repeatability is good. Since 1980, the 20th edition of the United States Pharmacopoeia included radon trials, pharmacopoeia and other countries have successively included this method.
Creative BioMart offers a corresponding endotoxin testing service. You can purchase the corresponding endotoxin removal kit and related accessory products according to the needs of your own samples. We guarantee that all instruments, water, reagents, and consumables used in the experiment are free of endotoxins, and the experiment is conducted in a clean room to ensure that low levels of endotoxins are returned to the sample. In addition, we can also provide you with related services including:
|Project name||Clinically relevant endotoxin testing|
|Testing purpose||In the clinic, there are many situations that require endotoxin testing, otherwise it will cause serious consequences. For example, in the sterilization process of the hemodialysis equipment system, the dialysis water exceeding the endotoxin level, the dialysis concentration and the dialysate will bring the endotoxin into the blood of uremic patients, thereby causing endotoxemia in uremic patients.|
|Testing cycle||3-5 days.|
|Service including||We provide you with raw data and test reports.|