Endotoxin Testing by Recombination Factor C (rFC) Assay

Mechanism of rFC

Recombination factor C detection (rFC) detection is an evolutionary version of LAL/TAL detection developed by combining recombination techniques with the LAL/TAL mechanism of action. Recombination factor C is activated by endotoxin to cleave the fluorescent substrate, and the presence or absence and intensity of the fluorescent signal are used to determine the presence or absence of endotoxin.[1]

The mechanism of rCF is as follows:

Endotoxin Testing by Recombination Factor C (rFC) Assay

Method of rFC

The recombination factor C in the rFC assay is activated by endotoxin, followed by direct cleavage of the artificially added fluorescent substrate to form a fluorescent product. Finally, we determine the amount of endotoxin by detecting fluorescence.

Advantages of rFC

Compared to the multi-step enzymatic process required for LAL/TAL assays, the rFC assay works by a single enzymatic step, which is faster and easier. Moreover,

Creative BioMart offers the Recombination Factor C (rFC) Assay service for a wide range of samples and endotoxin testing to meet your diverse endotoxin testing needs. And we can offer:

Creative BioMart offers the Recombination Factor C (rFC) Assay service for fast and accurate detection of bacterial endotoxins. We have a team of professional and well-equipped scientists dedicated to working with researchers around the world to meet customer requirements and ensure quality of service. If you are interested in our services, please contact us for more details.

Reference

  1. Loverock, B, et al. A recombination factor C procedure for the detection of Gram-negative bacterial endotoxin [J/OL]. Pharmacopeial Forum, 2010, 36(1):321 [2012-10-23].

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