Thrombin is a serine protease, an enzyme encoded by the F2 gene in humans. Thrombin (coagulation factor II) is proteolytically cleaved during the coagulation process to form thrombin. Thrombin in turn acts as a serine protease, which converts soluble fibrinogen into insoluble fibrin chains and catalyzes many other coagulation-related reactions. In the coagulation pathway, the function of thrombin is to convert factor XI to XIa, VIII to VIIIa, V to Va, fibrinogen to fibrin, and XIII to XIIIa. Thrombin is an important clinical hemostatic drug, with a large market demand and broad application prospects. However, most of the thrombin products produced by most companies currently have more protein impurities, higher endotoxin content, lower stability, and clinical accidents occur from time to time. Therefore, the detection of impurity proteins in thrombin products, the study of the method of removing endotoxin in thrombin products and the effects of additives on the stability of thrombin solution can provide a basis for the optimization of production processes, the removal of impurity proteins, and the improvement of product safety and stability.
Fig1. Protein structure of Thrombin.
Endotoxin removal is one of the most difficult tasks downstream in protein purification. At Creative BioMart, our experienced scientists will develop a customized protocol for your target protein after estimating endotoxin removal efficiency and sample recovery. We will use a combination of the following methods to ensure maximum efficiency.
The hydrophobic column interacts with nonpolar protein surfaces through van der Waals forces for endotoxin removal.
It uses composite polyacrylamide as the column, which is highly porous. Ultrafiltration results in relatively good endotoxin clearance from product solutions when the products are of low molecular weight.
This method involves the separation of aqueous surfactant solution into micelle-rich and micelle-poor regions through excluded-volume interactions. External agent notably Triton X-114 is needed to maintain the inherent biological activity of the protein while reducing endotoxin level by 100-fold.
The high selectivity of affinity chromatography eliminates the need for multiple purification steps and reduces production costs. At the same time, this method can remove endotoxin with high specificity and efficiency, resulting in excellent target recovery.
Creative BioMart offers a corresponding endotoxin testing service. You can purchase the corresponding endotoxin removal kit and related accessory products according to the needs of your own samples. We guarantee that all instruments, water, reagents, and consumables used in the experiment are free of endotoxins, and the experiment is conducted in a clean room to ensure that low levels of endotoxins are returned to the sample. In addition, we can also provide you with related services including:
|Project name||Endotoxin removal services in thrombin|
|Removal cycle||5-7 days.|
|Removal purpose||Most thrombin products currently produced by most companies have high endotoxin content, and clinical accidents sometimes occur. Therefore, the detection of impurity proteins in thrombin products, the study of the method of removing endotoxin in thrombin products and the influence of additives on the stability of thrombin solution can be used to optimize the production process, remove impurity proteins, and improve product safety and stability.|
|Service including||Creative BioMart can screen for the source of endotoxin contamination in the production process and propose several solutions to remove endotoxin to reduce the endotoxin content in the finished product.|