Endotoxin Testing by Monocyte Activation Test (MAT)

Mechanism of MAT

The MAT is developed based on the in vitro activation of human monocytoid cells by pyrogens. The proinflammatory cytokines, for example tumour necrosis factor alpha (TNFα), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6), that released from the activation are determined by ELISA.[1]

The mechanism of MAT is as follows:

Endotoxin Testing by Monocyte Activation Test (MAT)

Method of MAT 

A. Human whole blood incubation

The sample was mixed with fresh human blood at a certain ratio of volume. The reaction tubes were shaken gently and incubated 16-24 hr at 37℃. After that the tubes were shaken again, centrifuged and stored supernatants containing cytokine at -80℃.[2]

B. Enzyme-linked immunosorbent assay (ELISA)

Cytokine measurements were carried out with an in-house sandwich ELISA. According to the plot of endotoxin standard concentration (from 0.03125 to 2 EU/ml) vs. absorbance, the MAT response to endotoxin in samples.

Advantages of MAT

Our Service

For MAT method, our advantages are:

Creative BioMart offers the Monocyte Activation Test (MAT) assay service for fast and accurate detection of bacterial endotoxins. We have a team of professional and well-equipped scientists dedicated to working with researchers around the world to meet customer requirements and ensure quality of service. If you are interested in our services, please contact us for more details.

References

  1. Rolando Predomo-Morales, et al. Monocyte Activation Test (MAT) Reliable Dectects Pyrogens in Parenteral Formulations of Human Serum Albumin. ALTEX 28. 2011, 3-11.
  2. Andrade, S. et al. Comparative evaluation of eh human whole blood and human peripheral blood monocyte tests for pyrogens. Int. J. Pharm 265. 2003. 115-124
  3. Montag, T. et al. Safety testing of cell-based medicinal products: opportunities for the monocyte activation test for pyrogens. ALTEX 24, 2007, 81-89.

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