The MAT is developed based on the in vitro activation of human monocytoid cells by pyrogens. The proinflammatory cytokines, for example tumour necrosis factor alpha (TNFα), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6), that released from the activation are determined by ELISA.
The mechanism of MAT is as follows:
A. Human whole blood incubation
The sample was mixed with fresh human blood at a certain ratio of volume. The reaction tubes were shaken gently and incubated 16-24 hr at 37℃. After that the tubes were shaken again, centrifuged and stored supernatants containing cytokine at -80℃.
B. Enzyme-linked immunosorbent assay (ELISA)
Cytokine measurements were carried out with an in-house sandwich ELISA. According to the plot of endotoxin standard concentration (from 0.03125 to 2 EU/ml) vs. absorbance, the MAT response to endotoxin in samples.
For MAT method, our advantages are:
B. Medicine devices
D. Biological products
Creative BioMart offers the Monocyte Activation Test (MAT) assay service for fast and accurate detection of bacterial endotoxins. We have a team of professional and well-equipped scientists dedicated to working with researchers around the world to meet customer requirements and ensure quality of service. If you are interested in our services, please contact us for more details.