Peritoneal fluid is a fluid produced in the abdominal cavity that lubricates the tissue surface of the abdominal wall and the inner wall of the pelvic cavity. It covers most of the organs of the abdomen. Increased ascites is called ascites. Peritoneal fluid sampling is usually performed by puncture. Spontaneous infection of peritoneal effusion can cause spontaneous bacterial peritonitis (SBP) and endotoxemia. Intestinal bacterial overgrowth and intestinal wall permeability increase in patients with liver cirrhosis, and the local immune defense function of the intestinal wall is reduced, causing intestinal bacteria to transfer into the circulatory system through mesenteric lymph nodes, resulting in bacteremia. The peritoneal effusion is caused by the weakened activity of the reticuloendothelial system and the decline of opsonin, immunoglobulin, complement and albumin. Because endotoxin is part of the cell wall of Gram-negative bacteria, it is usually absorbed from the gastrointestinal tract into portal vein blood and cleared by the reticuloendothelial cells of the liver. In patients with severely damaged liver or overt portal hypertension, endotoxins can appear in ascites or systemic circulation if they are not infected.
The rabbit pyrogen test is based on the biological characteristics of endotoxin pyrogenicity. The body temperature of rabbits injected with the side sample was observed to determine the endotoxin in the prescribed time. The rabbit pyrogen test method has been mainly used for drug endotoxin testing. The force method has low sensitivity and has been used less frequently.
LAL test is a kind of arthropod. Amebocytes in the blood contain factor C, factor B and proclottin-gEnzyme, which can be activated by endotoxin. The activated coagulase can make the coagulation in the deformed cells coagulagen is coagulated to form coagulated proteins, which can be used for qualitative, semi-quantitative and quantitative determination of endotoxin. The reaction has high sensitivity (pg/ml) and specificity. It can be divided into gel method, turbidity method and color matrix method.
Creative BioMart offers a corresponding endotoxin testing service. You can purchase the corresponding endotoxin removal kit and related accessory products according to the needs of your own samples. We guarantee that all instruments, water, reagents, and consumables used in the experiment are free of endotoxins, and the experiment is conducted in a clean room to ensure that low levels of endotoxins are returned to the sample. In addition, we can also provide you with related services including:
|Project name||Endotoxin testing in peritoneal effusion|
|Testing purpose||In patients with severely damaged liver or obvious portal hypertension, if not infected, endotoxin will appear in ascites or systemic circulation. Spontaneous infection of peritoneal effusion can cause spontaneous bacterial peritonitis (SBP) and endotoxemia.|
|Testing cycle||3-5 days.|
|Service including||We provide you with raw data and test reports.|