Direct structural identification of intact lipopolysaccharides not only saves time, but also avoids structural changes during processing. However, amphiphilic lipopolysaccharides are easy to aggregate in solution, and the negatively charged groups on them can be cross-linked by divalent cations, which sets up obstacles for the identification of lipopolysaccharide structures.
The identification of intact lipopolysaccharides by mass spectrometry is mainly applicable to R-form lipopolysaccharides. With the development of MS, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) can also realize the efficient and fast detection of molecular mass and composition of intact lipopolysaccharides based on the establishment of sample preparation methods that overcome the amphipathic, low solubility and high charge characteristics of lipopolysaccharides. Reflectron matrix-assisted laser desorption ionization time-of-flight mass spectrometry (Reflectron MALDI-TOF MS) can also identify intact R-form lipopolysaccharides in the negative ion mode.
Creative BioMart has accumulated a lot of experience in LPS analysis, and has provided LPS-related services and products to a large number of researchers and companies around the world. After continuous improvement and upgrading, we have established an advanced mass spectrometry platform to comprehensively characterize endotoxins. On our platform, customers can choose different experimental schemes according to different needs, and our experts can also provide you with one-stop customized services.
Application: Improved characterization methods enable optimal results for LPS characterization within a variety of matrices.
Time: Less than 4 weeks
Information: Obtain various structural information of LPS (LPS profile, lipid A profile, fatty acid number, polysaccharide profile)
Applications: Screening of optimal antigens, structural validation for best quality results and validation of molecular species.
Time: Less than 5 weeks
Information: Best candidate screening, identification and validation of specific antigen structures, LSP structural information, Process improvements.
As a leading expert in endotoxin research, Creative BioMart has the solid expertise and scientific background to provide strong technical support to researchers around the world. To speed up your research process, we offer high-quality mass spectrometry services for LPS analysis. Our experts can also customize experiments to suit your needs, or offer cutting-edge research topics related to endotoxins. If you are interested in our services, please contact us for more details.
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