Escherichia coli, also known as Escherichia coli, is a Gram-negative, anaerobic, rod-shaped, Escherichia coli bacterium. It is usually found in the lower small intestine (endothermic) of warm-blooded creatures. Most E. coli strains are harmless, but certain serotypes can cause severe food poisoning in the host and occasionally cause food contamination events leading to product recalls. Harmless strains are part of the normal intestinal microbiota and can help blood coagulate by producing vitamin K2) and prevent the colonization of pathogenic bacteria in the intestine, thereby benefiting its host, which has a symbiotic relationship. E. coli is excreted into feces.
Fig1. Escherichia coli.
Significance for testing endotoxin in Eoil
Endotoxin (ET) is a lipopolysaccharide (LPS) in the outer membrane structure of the cell wall. It can cause various pathological changes and various complications such as fever, shock, and organ damage. Studies have shown that the toxic effects and clinical symptoms caused by LPS are roughly the same, and are mainly manifested in symptoms such as pyrogenicity, leukocytosis (small dose) or reduction (large dose), diffuse intravascular coagulation, and increased blood sugar. Very low concentrations of endotoxin can affect the normal physiological activities of certain cells and cause severe inflammatory reactions in humans or animals. Endotoxin in E. coli is a common pyrogen substance in infusion. The toxin is more resistant to heat. Therefore, it is of great significance to detect endotoxin in E. coli.
Creative BioMart's serum endotoxin test mainly uses limulus amebocyte lysate test, which is a kind of arthropod. Amebocytes in the blood contain factor C, factor B and proclottin-gEnzyme, which can be activated by endotoxin. The activated coagulase can make the coagulation in the deformed cells coagulagen is coagulated to form coagulated proteins, which can be used for qualitative, semi-quantitative and quantitative determination of endotoxin. The reaction has high sensitivity (pg/ml) and specificity. It can be divided into gel method, turbidity method and color matrix method.
To meet the challenging requirements, Creative BioMart has built a team of experienced scientists with facilities and processes designed specifically to offer corresponding endotoxin testing services. You can also purchase the corresponding endotoxin removal kit and related accessories according to your sample needs. We guarantee that all instruments, water, reagents, and consumables used in the experiment are free of endotoxins, and the experiment is conducted in a clean room to ensure that low levels of endotoxins are returned to the sample. In addition, we can also provide you with related services including:
|Project name||Endotoxin detection in E.coli|
|Testing purpose||Endotoxin (ET) can cause various pathological changes and various complications, such as fever, shock and organ damage. Therefore, endotoxins must be tested before use.|
|Testing cycle||3-5 days.|
|Service including||We provide you with raw data and test reports.|
Creative BioMart offers a corresponding endotoxin detection service. You can purchase the corresponding endotoxin removal kit and related accessory products according to the needs of your own samples. We guarantee that all instruments, water, reagents, and consumables used in the experiment are free of endotoxins, and the experiment is conducted in a clean room to ensure that low levels of endotoxins are returned to the sample. In addition, we can also provide you with related services including:
If you are interested in our services, please contact us for more details.