Application of Endotoxin Testing in Urinary Tract Infection

Endotoxin is the main component of the cell wall of Gram-negative bacteria, which can cause fever, microcirculation disorders, endotoxin shock, and disseminated intravascular coagulation. It can only be released after the bacteria die. Urinary tract infection is one of the common diseases of the urinary system. It is a disease caused by the proliferation of a large number of microorganisms in the urethra. If not treated promptly, it will cause chronic infection. Gram-negative bacteria are the main bacteria that cause urinary tract infections. As a gold standard for diagnosing urinary tract infections, urinary bacterial cultures can provide clinical observations of the number of clinically infected bacteria, drug sensitivity, and corresponding efficacy. However, urinary bacterial culture takes about 2 to 4 days, which cannot provide clinical treatment basis in time. Urine endotoxin testing can prompt Gram-negative bacterial infections and provide clinicians with reference information for diagnosis and treatment efficacy in a timely manner. Foreign reports indicate that when treated with antibacterial drugs, urine culture bacteria may not grow, but endotoxin is positive. Because endotoxins are released after bacterial death during antimicrobial treatment, the determination of endotoxin may be more sensitive than bacterial culture. This topic compares the level of endotoxin in urine with the results of urinary bacterial cultures, and studies its diagnostic value in urinary tract infections in order to find an early and accurate diagnostic method for urinary tract infections to guide the curative effect.

Creative BioMart uses Limulus Amebocyte Lysate test for urine endotoxin testing. LAL test is a kind of arthropod. Amebocytes in the blood contain factor C, factor B and proclottin-gEnzyme, which can be activated by endotoxin. The activated coagulase can make the coagulation in the deformed cells coagulagen is coagulated to form coagulated proteins, which can be used for qualitative, semi-quantitative and quantitative determination of endotoxin. The reaction has high sensitivity (pg/ml) and specificity. It can be divided into gel method, turbidity method and color matrix method.


Gram-negative bacteria have been the main pathogens of urinary tract infections. At present, the test method for urinary tract infections is mainly urine culture, which has the advantages of identifying pathogenic bacteria and providing the results of drug sensitivity tests. However, its shortcomings are also obvious, that is, the culture time is relatively long, usually 2 to 4 days, it cannot provide timely test results, and the early clinical diagnosis and anti-infective treatment guidance are not timely enough. Urine culture also has requirements for submitting test specimens. Retention of urine specimens after using antibacterial drugs is also likely to cause false negative results. The determination of urine endotoxin has two advantages: fast speed and high sensitivity. It can prompt the infection of Gram-negative bacteria, and the results can be obtained in 4 to 5 hours, which can provide clinical doctors with reference information for diagnosis and treatment effect in time.

Creative BioMart offers a corresponding endotoxin testing service. You can purchase the corresponding endotoxin removal kit and related accessory products according to the needs of your own samples. We guarantee that all instruments, water, reagents, and consumables used in the experiment are free of endotoxins, and the experiment is conducted in a clean room to ensure that low levels of endotoxins are returned to the sample. In addition, we can also provide you with related services including:

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