Application of Endotoxin Testing in the Diagnosis of Chronic Obstructive Pulmonary Disease

Chronic obstructive pulmonary disease (COPD) has become the leading cause of death and disability in respiratory diseases. With the increasing unreasonable use of antibiotics, the pathogenic bacteria of COPD have changed significantly. Whether it is community-acquired infections or nosocomial infections, infections caused by G-bacteria have increased significantly. Although empirical anti-infection treatment can control some of the symptoms of infection, it is easy to cause the imbalance of the flora in the body, leading to the occurrence of double infection or nosocomial infection, and increasing the risk of pathogenic resistance. Therefore, the early diagnosis of pathogens in the clinic is particularly important, especially for the testing of bacterial endotoxins. However, at present, there is a lack of rapid and simple methods for examining pathogens.

On the left is a diagram of the lungs and airways with an inset showing a detailed cross-section of normal bronchioles and alveoli. On the right are lungs damaged by COPD with an inset showing a cross-section of damaged bronchioles and alveoli.Figure 1. On the left is a diagram of the lungs and airways with an inset showing a detailed cross-section of normal bronchioles and alveoli. On the right are lungs damaged by COPD with an inset showing a cross-section of damaged bronchioles and alveoli.

Discussion

Endotoxin is a component of lipopolysaccharide released by G-bacteria during growth and disintegrating after death. The core of the toxicity of G-bacteria lipopolysaccharide is lipidoid A, and lipidoid A only releases G- Only bacteria can exert their toxic effects; therefore, endotoxin levels are roughly positively correlated with G-bacteria. Due to the presence of the human immune system and the use of clinical antibiotics, G-bacteria are killed and endotoxins are released. Therefore, quantitative testing of endotoxins can be used to judge the infection of G-bacteria at an early stage and provide a basis for clinical treatment. Compared with the pathogen culture, the endotoxin testing speed is fast and the positive rate is high, which can guide clinical medication early. Comparison of endotoxin testing with etiological examination can promptly and quickly indicate that AECOPD patients are G- infected or G+-infected. The endotoxin test is positive, and the analyzed patient may have mixed infection; it may also be related to the weakening of the intestinal mucosa shielding function of the body under the stress response state, promoting bacterial translocation and producing intestinal endotoxemia.

Creative BioMart offers a corresponding endotoxin testing service. You can purchase the corresponding endotoxin removal kit and related accessory products according to the needs of your own samples. We guarantee that all instruments, water, reagents, and consumables used in the experiment are free of endotoxins, and the experiment is conducted in a clean room to ensure that low levels of endotoxins are returned to the sample. In addition, we can also provide you with related services including:

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