Application of Cerebrospinal Fluid Endotoxin Testing in Diagnosis of Meningitis in Children

Intracranial infections in children mainly include viral encephalitis (sick brain), purulent meningitis (chemical brain), tuberculous meningitis, and cryptococcal meningitis. Among them, diseased and chemical brain are the most common. At present, Gram-negative bacilli, especially Escherichia coli, in children's encephalopathy have been increasing year by year. Bacterial endotoxin is a unique structure on the cell wall of Gram-negative bacilli. Its main chemical component is lipopolysaccharide, which is released when the cell wall disintegrates after the death of the bacteria. It can also be released by live bacteria. Testing of cerebrospinal fluid (CSF) bacterial endotoxin is of great significance in the early diagnosis and efficacy evaluation of children with Gram-negative meningitis.

General testing methods

CSF and serum samples were collected before using antibiotics within 24 hours after admission. In the Huanao group, CSF and serum samples were collected at 1 and 2 weeks of regular antibiotic treatment, respectively. The method for detecting bacterial endotoxin is as follows: take 0.2mL of the sample to be tested and directly add it to the main enzyme reaction agent; after dissolving, transfer it to a 9mm × 65mm standard pyrogen-free flat-bottom test tube and insert it into the MB80 microbe rapid dynamic testing system After the reaction, the testing system automatically calculates the bacterial endotoxin content in the test sample.

Discussion

Bacterial endotoxin exists in the cell wall of Gram-negative bacteria, and is released when the cell wall disintegrates after bacterial death. Live bacteria can also release endotoxin in the form of blisters. Since the establishment of the limulus test method, the application of bacterial endotoxin testing in clinical testing has developed rapidly. With the continuous improvement of test method research and measuring instruments, the testing of bacterial endotoxins has been miniaturized and accelerated. Trace amounts of bacterial endotoxins in body fluids can be detected by the limulus amebocyte lysate test (LAL). There have been reports of quantitative bacterial culture of bronchoalveolar lavage fluid and determination of endotoxin, and found that the level of endotoxin is proportional to the number of Gram-negative bacilli. It has been reported that bacterial endotoxin testing in patients with Gram-negative bacillus peritonitis and continuous ambulatory peritoneal dialysis believes that endotoxin testing is a rapid and sensitive method that can make an early judgment on the type of bacteria infected and the presence of endotoxemia. Conducive to empirical treatment of clinical diseases.

Creative BioMart offers a corresponding endotoxin testing service. You can purchase the corresponding endotoxin removal kit and related accessory products according to the needs of your own samples. We guarantee that all instruments, water, reagents, and consumables used in the experiment are free of endotoxins, and the experiment is conducted in a clean room to ensure that low levels of endotoxins are returned to the sample. In addition, we can also provide you with related services including:

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