| Cat#: | EKIT-016 |
| Description/Features: | The Endotoxin Plate Kit is a kinetic, colorimetric assay for the quantitative determination of bacterial Endotoxin in aqueous solutions. The detection ranges from 0.01 - 10 Endotoxin units (EU/mL).The test is based upon an enzymatic cascade where Endotoxin activates Factor C in Limulus Amebocyte Lysate (LAL) which in turn activates Factor B. Factor B activates Proclotting Enzyme which then activates Clotting Enzyme. A colorless synthetic peptide substrate is hydrolyzed by Clotting Enzyme to generate a yellow color which can be measured by a spectrophotometer at 405 nm. The degree of color resulting from the reaction is proportional to the amount of Endotoxin in the test sample and can be calculated using a standard curve. |
| Capacity: | The detection ranges from 0.01 - 10 Endotoxin units (EU/mL). |
| Package: | 96-well plate |
| Application: | Endotoxin level detection |
| Storage: | Store all kit reagents at 2-8 °C in the dark. Avoid storing kits for extended periods (more than 24 hours) at room temperature. |
| Handling: | Wet Ice |
| Key words: | Endotoxin Test Kit, Endotoxin level kit, Endotoxin Detection, bacterial Endotoxin, Limulus Amebocyte Lysate (LAL) |
