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Storage: Store at 4°C for two years. The endotoxin test reagent should be properly divided immediately after dissolution, and stored at -20°C for one week, and at least two months after being frozen at -80°C in liquid nitrogen. The remaining reagents are dissolved and stored at -20°C for at least 6 months.
Principle: Limulus reagents are Lyophilized Amebocyte Lysate (LAL) or similar reagents reconstituted in vitro. Limulus reagents contain C factor, B factor, coagulase, coagulinogen, etc. Under suitable conditions (temperature, pH, and no interfering substances), the endotoxin activates factor C, causing a series of enzymatic reactions that activate the coagulase procoagulase to form coagulase, which breaks down the chromogens provided in this kit into polypeptides and yellow p-nitroaniline (pNA, λmax =405nm). In a certain time, the production of pNA was positively correlated with the concentration of endotoxin. At the same time, p-nitroaniline (pNA) can also react with the azotization reagent to produce rose red product (λmax =545nm), according to which the endotoxin concentration of the sample to be measured can be quantified, and the color of the sample to be measured can avoid interference with the absorption peak at 405nm.
