Recombinant Factor C Endpoint Fluorescent Assay

The Recombinant Factor C Assay is the evolution of endotoxin detection testing. Our scientists have developed a recombinant form of Factor C, the first component in the horseshoe crab clotting cascade activated by endotoxin. Recombinant Factor C (rFC) is activated by endotoxin binding, and the active enzyme then cleaves a synthetic substrate, resulting in the generation of a fluorogenic compound. rFC works through a single enzymatic step compared to the multiple step enzymatic process necessary for LAL assays. The reaction is run in a 96-well microplate and is measured at time zero and after a one-hour incubation in a fluorescent microplate reader using excitation/emission wavelengths of 380/440 nm. In the presence of endotoxin, activated rFC will cleave the fluorogenic substrate, causing the solution to fluoresce. The log net fluorescence is proportional to the log endotoxin concentration and is linear in the 0.005 - 5.0 EU/ml range.

CATALOG NUMBER PRODUCT Package Storage Inquiry
EKIT-006 Recombinant Factor C Endpoint Fluorescent Assay 2,880 tests INQUIRY
EKIT-006 Recombinant Factor C Endpoint Fluorescent Assay 192 tests INQUIRY

Specification

Cat# EKIT-006
Application In-process testing Final product release testing Water testing Testing plant-based material
Product Sensitivity 0.005 to 5 EU/ml
Kit Components 30 x 96 tests/vial rFC enzyme solution 30 x 6 mL/vial fluorogenic substrate 30 x 5 mL/vial rFC assay buffer 10 vials endotoxin
Highlights Higher endotoxin specificity through the elimination of false positive glucan reactions Less lot-to-lot variability Security of supply FDA acknowledged alternative to LAL
Usage For Research Use Only! Not For Use in Humans.

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