HGF, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. It can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions.
| Short Name: |
Recombinant Mouse Hgf protein, His-tagged, low endotoxin |
| Cat#: |
EPLE-17M |
| Recombinant: |
Human Cells |
| Abbr: |
Hgf, His-tagged(Mouse) |
| Alias: |
HGF; hepatocyte growth factor; SF; scatter factor; hepatopoeitin-A; hepatopoietin-A; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik; |
| Type: |
H |
| similar: |
Hgf |
| Product Overview: |
Recombinant Mouse Hepatocyte growth factor is produced by our Mammalian expression system and the target gene encoding Gln33-Leu728 is expressed with a 6His tag at the C-terminus. |
| Source: |
Human Cells |
| Species: |
Mouse |
| Tag : |
His |
| Molecular Mass: |
Mol Mass: 53.5&26.9 kDa
AP Mol Mass: 51-59&32-38 kDa, reducing conditions. |
| Protein length: |
Gln33-Leu728 |
| Endotoxin: |
Less than 0.001 ng/μg (0.01 EU/μg) as determined by LAL test. |
| Purity: |
Greater than 95% as determined by reducing SDS-PAGE. |
| Storage: |
Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months. |
| Storage Buffer: |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution: |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Gene Name: |
Hgf hepatocyte growth factor [ Mus musculus ] |
| Official Symbol: |
Hgf |
| Synonyms: |
HGF; hepatocyte growth factor; SF; scatter factor; hepatopoeitin-A; hepatopoietin-A; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik; |
| GeneID: |
15234 |
| mRNA Refseq: |
NM_010427 |
| Protein Refseq: |
NP_034557 |
| UniProt ID: |
Q08048 |
| References: |
1. A self-assembling peptide hydrogel-based drug co-delivery platform to improve tissue repair after ischemia-reperfusion injury[J]. Shuyun Liu. et al. Acta Biomaterialia. 2019. |
| Figure 1 |
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