Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenic factor. It belongs to the peptidase S1 family and Plasminogen subfamily, contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. HGF regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. HGF is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration.
| Short Name: |
Recombinant Human HGF protein, His-tagged, low endotoxin |
| Cat#: |
EPLE-16H |
| Recombinant: |
Human Cells |
| Abbr: |
HGF, His-tagged(Human) |
| Type: |
H |
| Product Overview: |
Recombinant Human Hepatocyte Growth Factor is produced by our Mammalian expression system and the target gene encoding Gln32-Ser728 is expressed with a 6His tag at the C-terminus. |
| Source: |
Human Cells |
| Species: |
Human |
| Tag : |
His |
| Bio-activity: |
Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. The ED50 for this effect is 0.3-1.5 ng/mL. |
| Molecular Mass: |
Mol Mass: 26&53.7 kDa
AP Mol Mass: 32-38&55-65 kDa, reducing conditions. |
| Protein length: |
Gln32-Ser728 |
| Endotoxin: |
Less than 0.001 ng/μg (0.01 EU/μg) as determined by LAL test. |
| Purity: |
Greater than 95% as determined by reducing SDS-PAGE. |
| Storage: |
Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months. |
| Storage Buffer: |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, pH 8.0. |
| Reconstitution: |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Gene Name: |
HGF hepatocyte growth factor (hepapoietin A; scatter factor) [ Homo sapiens ] |
| Official Symbol: |
HGF |
| Synonyms: |
HGF; hepatocyte growth factor (hepapoietin A; scatter factor); deafness, autosomal recessive 39, DFNB39; hepatocyte growth factor; F TCF; fibroblast derived tumor cytotoxic factor; hepatopoietin A; HGFB; HPTA; lung fibroblast derived mitogen; scatter factor; SF; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor; F-TCF; DFNB39; |
| GeneID: |
3082 |
| mRNA Refseq: |
NM_000601 |
| Protein Refseq: |
NP_000592 |
| MIM: |
142409 |
| UniProt ID: |
P14210 |
| References: |
1. Aging Reprograms the Hematopoietic-Vascular Niche to Impede Regeneration and Promote Fibrosis[J]. Yutian Chen. et al. Cell Metabolism. 2021.
2. A Nongenetic Proximity-Induced FRET Strategy Based on DNA Tetrahedron for Visualizing the Receptor Dimerization[J]. Yanhua L. et al. Anal. Chem..2020. |
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| Figure 2 |
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