Glial Cell Line-Derived Neurotrophic Factor (GDNF) is a disulfide-linked homodimeric glycoprotein that belongs to the TGF-β superfamily. It has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. Mutations in this gene may be associated with Hirschsprung disease.
| Short Name: |
Recombinant Human GDNF Protein, low endotoxin |
| Cat#: |
EPLE-15H |
| Recombinant: |
E. coli |
| Abbr: |
GDNF(Human) |
| Alias: |
GDNF; glial cell derived neurotrophic factor; glial cell line-derived neurotrophic factor; astrocyte derived trophic factor; ATF1; ATF2; glial cell line derived neurotrophic factor; glial derived neurotrophic factor; HFB1 GDNF; ATF; astrocyte-derived trophic factor; HSCR3; HFB1-GDNF; |
| Type: |
G |
| similar: |
GDNF |
| Product Overview: |
Recombinant Human Glial Cell Line-Derived Neurotrophic Factor is produced by our E.coli expression system and the target gene encoding Ser78-Ile211 is expressed. |
| Source: |
E.coli |
| Species: |
Human |
| Molecular Mass: |
Mol Mass: 15.1 kDa
AP Mol Mass: 17 kDa, reducing conditions. |
| Protein length: |
Ser78-Ile211 |
| Endotoxin: |
Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. |
| Purity: |
Greater than 95% as determined by reducing SDS-PAGE. |
| Storage: |
Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months. |
| Storage Buffer: |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Reconstitution: |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Gene Name: |
GDNF glial cell derived neurotrophic factor [ Homo sapiens ] |
| Official Symbol: |
GDNF |
| Synonyms: |
GDNF; glial cell derived neurotrophic factor; glial cell line-derived neurotrophic factor; astrocyte derived trophic factor; ATF1; ATF2; glial cell line derived neurotrophic factor; glial derived neurotrophic factor; HFB1 GDNF; ATF; astrocyte-derived trophic factor; HSCR3; HFB1-GDNF; |
| GeneID: |
2668 |
| mRNA Refseq: |
NM_000514 |
| Protein Refseq: |
NP_000505 |
| MIM: |
600837 |
| UniProt ID: |
P39905 |
| References: |
1. Clinical development and evaluation of a VEGF-D assay in plasma from patients with metastatic colorectal cancer in the RAISE study[J]. Hiroya Taniguchi. et al. CURRENT MEDICAL RESEARCH AND OPINION. 2021. |
| Figure 1 |
|
