Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites.
| Short Name: |
Recombinant Human EGF Protein, low endotoxin |
| Cat#: |
EPLE-06H |
| Recombinant: |
E. coli |
| Abbr: |
EGF(Human) |
| Alias: |
EGF; epidermal growth factor; epidermal growth factor (beta urogastrone); pro-epidermal growth factor; beta-urogastrone; URG; HOMG4; |
| Type: |
E |
| similar: |
EGF |
| Product Overview: |
Recombinant Human Epidermal Growth Factor is produced by our E.coli expression system and the target gene encoding Asn971-Arg1023 is expressed. |
| Source: |
E.coli |
| Species: |
Human |
| Bio-activity: |
Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 60-450 pg/mL. |
| Molecular Mass: |
Mol Mass: 6.2 kDa
AP Mol Mass: 11 kDa, reducing conditions. |
| Protein length: |
Asn971-Arg1023 |
| Endotoxin: |
Less than 0.001 ng/μg (0.01 EU/μg) as determined by LAL test. |
| Purity: |
Greater than 95% as determined by reducing SDS-PAGE. |
| Storage: |
Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months. |
| Storage Buffer: |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 200mM NaCl, pH 8.0. |
| Reconstitution: |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Gene Name: |
EGF epidermal growth factor [ Homo sapiens ] |
| Official Symbol: |
EGF |
| Synonyms: |
EGF; epidermal growth factor; epidermal growth factor (beta urogastrone); pro-epidermal growth factor; beta-urogastrone; URG; HOMG4; |
| GeneID: |
1950 |
| mRNA Refseq: |
NM_001178130 |
| Protein Refseq: |
NP_001171601 |
| MIM: |
131530 |
| UniProt ID: |
P01133 |
| References: |
1. Protein tyrosine phosphatase SHP2 suppresses host innate immunity against influenza A virus through regulating EGFR-mediated signalings[J]. Qingsen Wang. et al. Journal of Virology. 2021.
2. SIDT1-dependent absorption in the stomach mediates host uptake of dietary and orally administered microRNAs[J]. Qun Chen. et al. Cell Research. 2020.
3. Loss of ELF5–FBXW7 stabilizes IFNGR1 to promote the growth and metastasis of triple-negative breast cancer through interferon-γ signalling[J]. Snahlata Singh. et al. NATURE CELL BIOLOGY. 2020. |
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