Dickkopf-related protein 1(DKK-1), is a member of the dickkopf family. DKK1 secreted proteins with two cysteine-rich domains separated by a linker region. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
| Short Name: |
Recombinant Human DKK1 protein, His-tagged, low endotoxin |
| Cat#: |
EPLE-05H |
| Recombinant: |
Human Cells |
| Abbr: |
DKK1, His-tagged(Human) |
| Type: |
D |
| Product Overview: |
Recombinant Human Dickkopf-related protein 1 is produced by our Mammalian expression system and the target gene encoding Thr32-His266 is expressed with a 8His tag at the N-terminus. |
| Source: |
Human Cells |
| Species: |
Human |
| Tag : |
His |
| Molecular Mass: |
Mol Mass: 27 kDa
AP Mol Mass: 42-47 kDa, reducing conditions. |
| Protein length: |
Thr32-His266 |
| Endotoxin: |
Less than 0.001 ng/μg (0.01 EU/μg) as determined by LAL test. |
| Purity: |
Greater than 95% as determined by reducing SDS-PAGE. |
| Storage: |
Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months. |
| Storage Buffer: |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution: |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Gene Name: |
DKK1 dickkopf 1 homolog (Xenopus laevis) [ Homo sapiens ] |
| Official Symbol: |
DKK1 |
| Synonyms: |
DKK1; dickkopf 1 homolog (Xenopus laevis); dickkopf (Xenopus laevis) homolog 1; dickkopf-related protein 1; DKK 1; SK; hDkk-1; dickkopf-1 like; dickkopf related protein-1; DKK-1; |
| GeneID: |
22943 |
| mRNA Refseq: |
NM_012242 |
| Protein Refseq: |
NP_036374 |
| MIM: |
605189 |
| UniProt ID: |
O94907 |
| Figure 1 |
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