Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family. Along with other structurally related neurotrophic factors NGF, NT-3 and NT-4, BDNF binds with high affinity to the TrkB kinase receptor. It also binds with the LNGFR (for low-affinity nerve growth factor receptor, also known as p75). BDNF promotes the survival, growth and differentiation of neurons. It serves as a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. BDNF expression is altered in neurodegenerative disorders such as Parkinson's and Alzheimer's disease.
| Short Name: |
Recombinant Human BDNF Protein, low endotoxin |
| Cat#: |
EPLE-03H |
| Recombinant: |
E. coli |
| Abbr: |
BDNF(Human) |
| Alias: |
BDNF; brain-derived neurotrophic factor; neurotrophin; abrineurin; MGC34632; |
| Type: |
B |
| similar: |
BDNF |
| Product Overview: |
Recombinant Human Brain-Derived Neurotrophic Factor is produced by our E.coli expression system and the target gene encoding His129-Arg247 is expressed. |
| Source: |
E.coli |
| Species: |
Human |
| Bio-activity: |
Immobilized Human TrkB-His at 2 μg/mL (100 μL/well) can bind Human BDNF: Biotinylated by NHS-biotin prior to testing. The ED50 of Human BDNF is 47.58 ng/mL. |
| Molecular Mass: |
Mol Mass: 13 kDa
AP Mol Mass: 14 kDa, reducing conditions. |
| Protein length: |
His129-Arg247 |
| Endotoxin: |
Less than 0.001 ng/μg (0.01 EU/μg) as determined by LAL test. |
| Purity: |
Greater than 95% as determined by reducing SDS-PAGE. |
| Storage: |
Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months. |
| Storage Buffer: |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2. |
| Reconstitution: |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Gene Name: |
BDNF brain-derived neurotrophic factor [ Homo sapiens ] |
| Official Symbol: |
BDNF |
| Synonyms: |
BDNF; brain-derived neurotrophic factor; neurotrophin; abrineurin; MGC34632; |
| GeneID: |
627 |
| mRNA Refseq: |
NM_001143805 |
| Protein Refseq: |
NP_001137277 |
| MIM: |
113505 |
| UniProt ID: |
P23560 |
| Figure 1 |
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| Figure 2 |
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