| Cat#: | E128 |
| Description/Features: | Lipid A derived from E. coli R515 (Re) LPS (biosynthetic) |
| Origin: | E. coli strain R515 |
| Forms: | Liquid. Colourless opaque aqueous solution. |
| Purity: | ≥99.9%. No detectable DNA, RNA and protein traces. |
| Concentration: | 1mg/ml (0.5mg/ml for 250µg size) stabilised in sterile, double-distilled water (ddWater), without any additives. |
| Biological Activity: | Optimal concentration is dependent upon cell type, species, desired activation and analysis: 0.1-1.0μg/ml. Does not activate any TLR other than TLR4 as tested up to 50μg/ml in relevant cellular systems (macrophages). |
| Usage: | Do not freeze. Ready-made solution is cell culture-grade. Prepare diluted Lipid A working solutions in water just prior to use, keep sterile. To yield a 100μg/ml (1,000-100x) stock solution add 100μl of Lipid A to 900μl endotoxin-free and sterile ddWater (not PBS) and mix well. |
| Storage: | Stable for at least 2 years after receipt when stored at +4°C. |
