| Cat#: | E119 |
| Description/Features: | Lipopolysaccharide (LPS) from S. minnesota strain R60 (Ra), R-type (rough/mutant) LPS |
| Origin: | S. minnesota strain R60 |
| Forms: | Liquid. Colourless clear aqueous solution. |
| Purity: | ≥99.9%. No detectable DNA, RNA and protein traces. |
| Concentration: | 1mg/ml stabilised in sterile, double-distilled water (ddWater), without any additives. |
| Biological Activity: | Optimal concentration is dependent upon cell type, species, desired activation and analysis: 0.05-1.0μg/ml. Does not activate any TLR other than TLR4 as tested up to 50μg/ml in relevant cellular systems (macrophages). |
| Usage: | Do not freeze. To yield a 100μg/ml (1,000-100x) stock solution add 100μl of LPS to 900μl endotoxin-free and sterile ddWater or 0.9% NaCl Solution and mix well. Prepare diluted LPS working solutions just prior to use, keep sterile. Ready-made solution is cell culture-grade. |
| Storage: | Stable for at least 2 years after receipt when stored at +4°C. |
