Cat#:	E116
Description/Features:	Lipopolysaccharide (LPS) from E. coli O55:B5, S-type (smooth/wild-type) LPS
Origin:	E. coli O55:B5
Forms:	Liquid. Colourless clear aqueous solution.
Purity:	≥99.9%. No detectable DNA, RNA and protein traces.
Concentration:	1mg/ml stabilised in sterile, double-distilled water (ddWater), without any additives.
Biological Activity:	Optimal concentration is dependent upon cell type, species, desired activation and analysis: 0.01-1.0μg/ml. Does not activate any TLR other than TLR4 as tested up to 50μg/ml in relevant cellular systems (macrophages).
Usage:	Do not freeze. Prepare diluted LPS working solutions just prior to use, keep sterile. Ready-made solution is cell culture-grade. To yield a 100μg/ml (1,000-100x) stock solution add 100μl of LPS to 900μl endotoxin-free and sterile ddWater (Cat. No.: IAX-900-002), 0.9% NaCl Solution (Cat. No.: IAX-900-003) or PBS (Cat. No.: IAX-900-001) and mix well.
Storage:	Stable for at least 2 years after receipt when stored at +4°C.