Cat#:	E114
Description/Features:	Lipopolysaccharide (LPS) from E. coli strain EH100 (Ra), R-type (rough/mutant) LPS
Origin:	E. coli strain EH100
Forms:	Liquid. Colourless clear aqueous solution.
Purity:	≥99.9%. No detectable DNA, RNA and protein traces.
Concentration:	1mg/ml stabilised in sterile, double-distilled water (ddWater), without any additives.
Biological Activity:	Optimal concentration is dependent upon cell type, species, desired activation and analysis: 0.05-1.0μg/ml. Does not activate any TLR other than TLR4 as tested up to 50μg/ml in relevant cellular systems (macrophages).
Usage:	Do not freeze. To yield a 100μg/ml (1,000-100x) stock solution add 100μl of LPS to 900μl endotoxin-free and sterile ddWater or 0.9% NaCl Solution and mix well. Prepare diluted LPS working solutions just prior to use, keep sterile. Ready-made solution is cell culture-grade.
Storage:	Stable for at least 2 years after receipt when stored at +4°C.