Endotoxin is the lipopolysaccharide (LPS) complex located in the outer membrane of gram-negative bacteria. During experimental procedures, large amount of endotoxins are shed and can easily contaminate labware, buffers and downstream products. Presence of endotoxin in any of these has a detrimental effect on culturing of cells and can lead to generation of irreproducible or negative experimental data. Therefore, it is critical to remove endotoxin from samples and products. Our Rapid Endotoxin Removal Regeneration Buffer is the endotoxin-free regeneration buffer containing 1% sodium deoxycholate. It is specially designed for quickly regenerating Rapid Endotoxin Removal Agarose, which can quickly and effectively eliminate endotoxins to < 0.05 EU/mL in solutions containing proteins or pharmacologically important components.