Endotoxin is the lipopolysaccharide (LPS) complex located in the outer membrane of gram-negative bacteria. During experimental procedures, large amount of endotoxins are shed and can easily contaminate labware, buffers and downstream products. Presence of endotoxin in any of these has a detrimental effect on culturing of cells and can lead to generation of irreproducible or negative experimental data. Therefore, it is critical to remove endotoxin from samples and products. Endotoxin Removal Equilibration Buffer is designed to equilibrate our Rapid Endotoxin Removal Agarose and High Capacity Endotoxin Removal Agarose to neutral pH for better detox efficiency and protein recovery. Both Rapid Endotoxin Removal Agarose and High Capacity Endotoxin Removal Agarose can effectively eliminate endotoxins to ≤ 0.05 EU/mL in solutions containing proteins or pharmacologically important components.