Protein A beads have been widely used for IgG purification. Maximum IgG specificity is achieved by eliminating its cell wall binding region, albumin binding region, and other non-specific regions. Endotoxin-Free Protein A Sepharose beads for IgG purification contain covalently coupled recombinant Protein A to 6% cross-linked Sepharose beads, the most popular resin for protein purification. Endotoxin-Free Protein A (Sepharose) column is designed for endotoxin-free IgG purification and immunoprecipitation. This column can rapidly purify IgG from serum, ascites and cell culture media from various species such as human, mouse, rat, goat and rabbit.