Lipopolysaccharide (LPS) Isolation Kit

The outer membrane of gram-negative bacteria contains lipopolysaccharide (LPS), a low molecular weight carbohydrate with a molecular mass of 10-20 kDa. It is heterogeneous, and composed of O antigen (a repeating glycan polymer), core oligosaccharide (which links the O antigen to Lipid A - the third component, and non-carbohydrate components such as phosphate and amino acids groups. Lipid A, has multiple fatty acids which serve to anchor LPS into the bacterial membrane allowing the O antigen and core oligosaccharide to protrude and contributes to a major part of the toxicity of gram-negative bacteria. Also known as endotoxin, when consumed by animals, LPS induces a strong inflammatory response and/or sepsis.This LPS Isolation Kit uses bacterial membrane lysis buffer and protein digestion to yield micrograms of LPS from bacterial culture (approximately 1-4% of dry weight). This kit does not use chlorofrom or phenol like traditional methods, and it will yield pure LPS in less than 2 hours that can be easily characterized and quantified!

CATALOG NUMBER PRODUCT Package Storage Inquiry
EKIT-002 Lipopolysaccharide (LPS) Isolation Kit 10 isolations -20°C INQUIRY

Specification

Cat# EKIT-002
Application Isolation of LPS from outer membrane of gram negative bacteria.
Kit_Components LPS Isolation Buffer Proteinase K (20 mg/ml)
Highlights This kit does not use chlorofrom or phenol like traditional methods Yields pure LPS in less than 2 hours that can be easily characterized and quantified
Storage -20°C
Shipping Condition Gel Packs
Usage For Research Use Only! Not For Use in Humans.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry

Enter Your Email Here to Subscribe.

© 2021 Creative BioMart. All rights reserved.