It is equipped with azo nitride dyeing agent, which has high sensitivity and can avoid the interference of the color of the colored specimen on the horseshoe crab test. It is equipped with an endotoxin-free test tube, which is convenient for incubation in a water bath. The test results can be read by a spectrophotometer or a common microplate reader. It can be applied to the sample background color is light yellow, such as plasma, serum samples.