The endotoxin is quantitatively detected by a color reaction of the guanidine reagent using a synthetic chromogenic substrate. Under suitable conditions (temperature, pH and non-interfering substances), bacterial endotoxin causes a series of enzymatic reactions, and coagulase decomposes the synthetic chromogenic substrate to decompose it into a polypeptide and yellow p-nitroaniline (pNA). , λmax = 405 nm); and within a certain period of time, the amount of pNA produced is positively correlated with the concentration of bacterial endotoxin, and the endotoxin concentration of the test sample is quantified accordingly.
