Mycoplasma Colorimetric LAMP Detection Kit, Red-to-Yellow Readout

Datasheet

Specification

Cat#:  MYD-037
Description/Features:  A colorimetric LAMP assay for direct mycoplasma DNA screening in cell culture supernatants and serum. UDG carryover control reduces contamination from prior amplification products.
Size:  25 or 100 tests
Application:  LAMP
Storage:  -20 °C, protect master mix from light
Product Stability:  1 Year
Limit of Detection:  20–200 copies/µL using positive-control plasmid
Specificity:  Detection coverage: Approximately 45 mycoplasma species reported
Kit Components:  For 25 tests:
2× Master mix with UDG: 250 µL
Polymerase: 25 µL
Positive control: 50 µL
Water: 200 µL
Mineral oil: 500 µl
Note:  Readout: Red-to-yellow color change
Carryover control: UDG
Target: Conserved 16S rRNA gene region
Reaction volume: 20 µL
Incubation: 37 °C for 5–10 minutes, then 61 °C for 45 minutes
For Research or Industrial Raw Materials, Not For Personal Medical Use!

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