| Cat#: | MYD-035 |
| Description/Features: | A hydrolysis probe assay for direct detection of mycoplasma DNA in culture supernatants and serum. An internal amplification control helps identify PCR inhibition. |
| Size: | 100 or 500 reactions at 20 µL |
| Storage: | -20 °C |
| Product Stability: | 1 Year |
| Limit of Detection: | Approximately 2–20 copies/µL, measured using the positive control |
| Specificity: | Detection coverage: 39 mycoplasma species reported |
| Kit Components: | For 100 tests: 2× Probe mix: 1 mL Primer/probe mix: 200 µL Internal control: 100 µL Positive control: 200 µL Water: 1 mL |
| Note: | Target: Conserved 23S rRNA operon DNA region Extraction: Not required for compatible culture supernatants and serum Detection channels: FAM target; VIC-compatible internal-control channel Reaction format: 20 µL in 96-well plates; 10 µL in 384-well plates |
