Endotoxin Affisorben consists of immobilized polymyxin B on 6B-CL. Polymyxins are a family of antibiotics that contain a cationic cyclopeptide with a fatty acid chain. Polymyxin B neutralizes the biological activity of endotoxins by binding to the lipid A portion of bacterial lipopolysaccharide. The immobilized polymixin B gel is a stable affinity matrix that resists the leaching of ligands into the valuable preparation.
| NaviID: | Endotoxin Affinity Filler |
| Cat#: | EAF10 |
| Category: | Endotoxin Affinity Filler |
| Form: | Supplied as slurry in 50% glycerol containing 0.02% sodium azide |
| Application: | Polymyxin B- 6B-CL is used in protein chromatography, affinity chromatography, and specialty resins. Polymyxin B- 6B-CL has been used to study periodontitis and endodontic infections as well as obesity and type 2 diabetes. Polymyxin B- 6B-CL is also used for the removal of endotoxin levels in protein solutions, cell culture media, and ascites. |
| Storage: | 4°C |
| Size: | 10 mL |
| Capacity: | 200 – 500 µg LPS from E. coli serotype 0128:B12 |
| Particle size: | 52 – 165 µm |
| pH Stability: | 3-10 |
| Matix: | 6B-CL (crosslinked agarose beads, 6%) |
| Ligand: | Polymyxin B |
