Endpoint Chromogenic Kit EC80545
Datasheet
Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) provides fast measurement for endotoxin quantification. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored item. Then the yellow item can further react with diazo reagents to form purple items with maximum absorbance at 545 nm. A regular spectrophotometer or microplate reader is required for the assay. The purple items are proportional to endotoxin concentration. Then endotoxin testing result is quantitative analysis.
Specification
NaviID: |
End-Point Chromogenic Endotoxin Assay |
Cat#: |
CEA02 |
Category: |
End-Point Chromogenic Endotoxin Assay |
: |
5 Lyophilized Amebocyte Lysate, 1.7ml/vial, 4 Water for BET, 50ml/vial, 5 CSE, 5 Chromogenic Substrate, 1.7ml/vial, 5 Diazo Reagent 1, 10ml/vial, 5 Diazo Reagent 2, 10ml /vial, 5 Diazo Reagent 3, 10ml/vial |
Application: |
Standard spectrophotometer with 540 – 545 nm filter is enough to quantify endotoxin with our kit. |
Sensitivity: |
0.01-0.1 EU/mL, 0.1-1 EU/m |
Notes: |
Lyophilized Amebocyte Lysate (LAL) reagent is made from amebocyte lysate derived blood of horseshoe crab. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.