| Cat#: | EKIT-037 |
| Description/Features: | Endotoxin removal kit is an endototoxin removal resin of high efficiency. It is based on affinity matrix of modified polymyxin B (PMB), which allows highly efficient endotoxin removal. The final endotoxin level can be decreased to less than 0.1 EU/ml with repeated use of the endotoxin removal resin. Final removal efficiency may vary depending on the sample type/source. |
| Form: | Kit |
| Package: | 1 Kit |
| Kit Composition: | Endotoxin Removal Resin; Regeneration Buffer(Sodium Phosphate-Buffer, pH 8.0; Triton-114); Equilibration Buffer(Phosphate-Buffer, pH 8.0); Flow-Speed Control; Collection Tubes; Tips (1 ml); Protocol. |
| Size: | 1.5 ml in pre-packed column |
| Capacity: | Up to 2,000,000 EU/ml resin |
| Particle size: | 90 μm |
| pH Stability: | pH 5 -10 |
| Support Matrix: | 4% cross-linked agarose, spherical beads |
| Ligand: | Modified PMB (Polymixin B) |
| Tolerable Ionic Strength: | 0.1 to 0.5 M NaCl |
| Application: | Endotoxin removes from purified recombinant proteins, peptides, antibodies, polysaccharides, etc. |
| Storage: | 2°C to 8°C (Do not freeze.) To regenerate the column, wash the column with 10 ml of equilibration buffer and allow the column to drain completely. Add ethanol to the regeneration buffer to a final concentration of 20%, adding at least 1.5 ml to the column for storage. |
| Notes: | Tested reagents that do not interfere with performance: 20% DMSO, 20% ethanol, 20% glycerol;1 M urea, 300 mM imidazole; 0.05% Tween 20, 10 mM DTT. |
