R-spondin-3 (RSPO3), also known as Protein with TSP type-1 repeat, Roof plate-specific spondin-3, Thrombospondin type-1 domain-containing protein 2, PWTSR, THSD2 and CRISTIN1, is a member of the thrombospondin type 1 repeat supergene family. RSPO3 is a secreted protein and widely expressed in many tissues. RSPO3 contains two Furin-like repeats which have been found in a variety of eukaryotic proteins involved in the mechanism of signal transduction by receptor tyrosine kinases, and one TSP type-1 domain, RSPO3 founctions as a activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Otherwise, RSPO3 may negatively regulate the TGF-beta pathway.
| Short Name: |
Recombinant Human RSPO3 protein, Fc/His-tagged, low endotoxin |
| Cat#: |
EPLE-25H |
| Recombinant: |
Human Cells |
| Abbr: |
RSPO3, Fc/His-tagged(Human) |
| Type: |
R |
| Product Overview: |
Recombinant Human R-spondin-3 is produced by our Mammalian expression system and the target gene encoding Gln22-Val201 is expressed with a Fc, 6His tag at the C-terminus. |
| Source: |
Human Cells |
| Species: |
Human |
| Tag : |
Fc/His |
| Molecular Mass: |
Mol Mass: 47.9 kDa
AP Mol Mass: 61 kDa, reducing conditions. |
| Protein length: |
Gln22-Val201 |
| Endotoxin: |
Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. |
| Purity: |
Greater than 95% as determined by reducing SDS-PAGE. |
| Storage: |
Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months. |
| Storage Buffer: |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution: |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Gene Name: |
RSPO3 R-spondin 3 [ Homo sapiens ] |
| Official Symbol: |
RSPO3 |
| Synonyms: |
RSPO3; R-spondin 3; R spondin 3 homolog (Xenopus laevis), thrombospondin, type I, domain containing 2, THSD2; R-spondin-3; FLJ14440; R-spondin 3 homolog; roof plate-specific spondin-3; protein with TSP type-1 repeat; thrombospondin, type I, domain containing 2; thrombospondin type-1 domain-containing protein 2; PWTSR; THSD2; CRISTIN1; |
| GeneID: |
84870 |
| mRNA Refseq: |
NM_032784 |
| Protein Refseq: |
NP_116173 |
| MIM: |
610574 |
| UniProt ID: |
Q9BXY4 |
| Figure 1 |
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