RSPO1 is a secreted protein,containing 2 FU(furin-like) repeats and 1 TSP type-1 domain and belonging to the R-spondin family. RSPO1 is required for the early development of gonads, regardless of sex. It has been found in mice only eleven days after fertilization. To induce cell proliferation, it acts synergistically with WNT4. They help stabilize β catenin, which activates downstream targets. RSPO1 is necessary in female sex development. It augments the WNT/β catenin pathway to oppose male sex development. In critical gonadal stages, between six and nine weeks after fertilization, the ovaries upregulate it while the testes downregulate it. RSPO1 can potentially aid in the treatment of mucositis, which is characterized by inflammation of the oral cavity. This unfortunate condition often accompanies chemotherapy and radiation in cancer patients with head and neck tumors.
| Short Name: |
Recombinant Human RSPO1 protein, His-tagged, low endotoxin |
| Cat#: |
EPLE-24H |
| Recombinant: |
Human Cells |
| Abbr: |
RSPO1, His-tagged(Human) |
| Alias: |
RSPO1; R-spondin 1; R spondin homolog (Xenopus laevis); R-spondin-1; FLJ40906; RSPONDIN; R-spondin homolog; roof plate-specific spondin-1; RSPO; CRISTIN3; RP11-566C13.1; |
| Type: |
R |
| similar: |
RSPO1 |
| Product Overview: |
Recombinant Human R-spondin-1 is produced by our Mammalian expression system and the target gene encoding Ser21-Ala263 is expressed with a 6His tag at the C-terminus. |
| Source: |
Human Cells |
| Species: |
Human |
| Tag : |
His |
| Bio-activity: |
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2-15 ng/mL. |
| Molecular Mass: |
Mol Mass: 27.8 kDa
AP Mol Mass: 40 kDa, reducing conditions. |
| Protein length: |
Ser21-Ala263 |
| Endotoxin: |
Less than 0.01 EU/μg as determined by LAL test. |
| Purity: |
Greater than 98% as determined by reducing SDS-PAGE. |
| Storage: |
Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months. |
| Storage Buffer: |
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
| Reconstitution: |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Gene Name: |
RSPO1 R-spondin 1 [ Homo sapiens ] |
| Official Symbol: |
RSPO1 |
| Synonyms: |
RSPO1; R-spondin 1; R spondin homolog (Xenopus laevis); R-spondin-1; FLJ40906; RSPONDIN; R-spondin homolog; roof plate-specific spondin-1; RSPO; CRISTIN3; RP11-566C13.1; |
| GeneID: |
284654 |
| mRNA Refseq: |
NM_001038633 |
| Protein Refseq: |
NP_001033722 |
| MIM: |
609595 |
| UniProt ID: |
Q2MKA7 |
| References: |
1. The CD39+ HBV surface protein-targeted CAR-T and personalized tumor-reactive CD8+ T cells exhibit potent anti-HCC activity[J]. Fan Zou. et al. Molecular Therapy. 2021.
2. Patient-derived tumor-like cell clusters for drug testing in cancer therapy[J]. Shenyi Yin. et al. Science Translational Medicine. 2020. |
| Figure 1 |
|
| Figure 2 |
|
