Pro-neuregulin-1,Neuregulin-1 beta 1（NRG1） is a single-pass type I membrane protein and belongs to the neuregulin family. It contains 1 EGF-like domain and 1 Ig-like C2-type (immunoglobulin-like) domain. Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. The protein concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development.
| Short Name: |
Recombinant Human NRG1 Protein, low endotoxin |
| Cat#: |
EPLE-22H |
| Recombinant: |
E. coli |
| Abbr: |
NRG1(Human) |
| Type: |
N |
| Product Overview: |
Recombinant Human Neuregulin-1 beta is produced by our E.coli expression system and the target gene encoding Ser2-Lys246 is expressed. |
| Source: |
E.coli |
| Species: |
Human |
| Molecular Mass: |
Mol Mass: 26.9 kDa
AP Mol Mass: 34 kDa, reducing conditions. |
| Protein length: |
Ser2-Lys246 |
| Endotoxin: |
Less than 0.001 ng/μg (0.01 EU/μg) as determined by LAL test. |
| Purity: |
Greater than 85% as determined by reducing SDS-PAGE. |
| Storage: |
Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months. |
| Storage Buffer: |
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
| Reconstitution: |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Gene Name: |
NRG1 neuregulin 1 [ Homo sapiens ] |
| Official Symbol: |
NRG1 |
| Synonyms: |
NRG1; neuregulin 1; HGL; pro-neuregulin-1, membrane-bound isoform; GGF; HRG; NDF; MSTP131; pro-NRG1; glial growth factor; neu differentiation factor; neuregulin 1 type IV beta 3; neuregulin 1 type IV beta 1a; sensory and motor neuron derived factor; heregulin, alpha (45kD, ERBB2 p185-activator); ARIA; GGF2; HRG1; HRGA; SMDF; MST131; |
| GeneID: |
3084 |
| mRNA Refseq: |
NM_001159995 |
| Protein Refseq: |
NP_001153467 |
| MIM: |
142445 |
| UniProt ID: |
Q02297 |
| References: |
1. Allosteric modulation of the catalytic VYD loop in Slingshot by its N-terminal domain underlies both Slingshot autoinhibition and activation[J]. Duxiao Yang. et al. The Journal of Biological Chemistry. 2018. |
| Figure 1 |
|
