Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs). Mouse Noggin cDNA encodes a 232 amino acid (aa) residue precursor protein with 19 aa residue putative signal peptide that is cleaved to generate the 213 aa residue mature protein which is secreted as a homodimeric glycoprotein. Secreted Noggin probably remains close to the cell surface due to its binding of heparin-containing proteoglycans. Noggin binds some BMPs such as BMP4 with high affinity and others such as BMP7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons.
| Short Name: |
Recombinant Mouse Nog protein, His-tagged, low endotoxin |
| Cat#: |
EPLE-21M |
| Recombinant: |
Human Cells |
| Abbr: |
Nog, His-tagged(Mouse) |
| Alias: |
NOG; noggin; |
| Type: |
N |
| similar: |
Nog |
| Product Overview: |
Recombinant Mouse Noggin is produced by our Mammalian expression system and the target gene encoding Gln28-Cys232 is expressed with a 6His tag at the C-terminus. |
| Source: |
Human Cells |
| Species: |
Mouse |
| Tag : |
His |
| Bio-activity: |
Measured by its ability to inhibit BMP-2-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells in the presence of 2 μg/mL of Recombinant Human/Mouse/Rat BMP-2. The ED50 for this effect is 2.1 μg/mL. |
| Molecular Mass: |
Mol Mass: 23.9 kDa
AP Mol Mass: 30 kDa, reducing conditions. |
| Protein length: |
Gln28-Cys232 |
| Endotoxin: |
Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. |
| Purity: |
Greater than 95% as determined by reducing SDS-PAGE. |
| Storage: |
Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months. |
| Storage Buffer: |
Lyophilized from a 0.2 μm filtered solution of PBS, 5mM EDTA, 5% Trehalose,pH7.4. |
| Reconstitution: |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Gene Name: |
Nog noggin [ Mus musculus ] |
| Official Symbol: |
Nog |
| Synonyms: |
NOG; noggin; |
| GeneID: |
18121 |
| mRNA Refseq: |
NM_008711 |
| Protein Refseq: |
NP_032737 |
| UniProt ID: |
P97466 |
| Figure 1 |
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| Figure 2 |
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