Recombinant Human LIF Protein, low endotoxin
Datasheet
Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF.
Specification
| Short Name: |
Recombinant Human LIF Protein, low endotoxin |
| Cat#: |
EPLE-18H |
| Recombinant: |
E. coli |
| Abbr: |
LIF(Human) |
| Alias: |
LIF; leukemia inhibitory factor; CDF; cholinergic differentiation factor; DIA; differentiation inhibitory activity; differentiation inducing factor; hepatocyte stimulating factor III; HILDA; human interleukin in DA cells; D factor; melanoma-derived LPL inhibitor; differentiation-inducing factor; hepatocyte-stimulating factor III; differentiation-stimulating factor; MLPLI; |
| Type: |
L |
| similar: |
LIF |
| Product Overview: |
Recombinant Human Leukemia Inhibitory Factor is produced by our E.coli expression system and the target gene encoding Ser23-Phe202 is expressed. |
| Source: |
E.coli |
| Species: |
Human |
| Bio-activity: |
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 25-150 pg/mL. |
| Molecular Mass: |
Mol Mass: 19.7 kDa
AP Mol Mass: 18 kDa, reducing conditions. |
| Protein length: |
Ser23-Phe202 |
| Endotoxin: |
Less than 0.001 ng/μg (0.01 EU/μg) as determined by LAL test. |
| Purity: |
Greater than 95% as determined by reducing SDS-PAGE. |
| Storage: |
Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months. |
| Storage Buffer: |
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
| Reconstitution: |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Gene Name: |
LIF leukemia inhibitory factor [ Homo sapiens ] |
| Official Symbol: |
LIF |
| Synonyms: |
LIF; leukemia inhibitory factor; CDF; cholinergic differentiation factor; DIA; differentiation inhibitory activity; differentiation inducing factor; hepatocyte stimulating factor III; HILDA; human interleukin in DA cells; D factor; melanoma-derived LPL inhibitor; differentiation-inducing factor; hepatocyte-stimulating factor III; differentiation-stimulating factor; MLPLI; |
| GeneID: |
3976 |
| mRNA Refseq: |
NM_001257135 |
| Protein Refseq: |
NP_001244064 |
| MIM: |
159540 |
| UniProt ID: |
P15018 |
| References: |
1. A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation[J]. Chao Sheng. et al. Nature Communications. 2018.
2. Determining Osteogenic Differentiation Efficacy of Pluripotent Stem Cells by Telomerase Activity[J]. Siqi Zhang. et al. Tissue Engineering and Regenerative Medicine. 2018.
3. Isolation of lung multipotent stem cells using a novel microfluidic magnetic activated cell sorting system[J]. L Zeng. et al. Cell Biology International. 2017. |
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| Figure 2 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
