This product is a resin used to remove endotoxin from biological source protein products (including peptides, antibodies, polysaccharides, etc.). The principle is to link the modified polymyxin B to 4% agarose microspheres for the specific removal of endotoxin. After purification, the endotoxin in the sample can be reduced to 0.1 EU/mL, and the sample recovery rate is high.
| NaviID: | Endotoxin Affinity Filler |
| Cat#: | EAF06 |
| Category: | Endotoxin Affinity Filler |
| Storage: | 4℃ |
| Size: | 10 mL |
| Capacity: | >2000000 EU/mL matrix |
| Particle size: | 45-165 μm |
| pH Stability: | 5-10 |
| Matix: | 4% Agarose microspheres |
| Ligand: | Modified polymyxin B |
| PressureMax: | 0.1 MPa, 1bar |
| Buffer: | 20% ethanol |
| Notes: | (1) Since the structure of endotoxin itself contains lipid chains, proteins, and polysaccharides, it is a very complex biological macromolecule, and it can be combined with your target molecule in the buffer solution through hydrophobic interactions and other methods. Therefore, in the process of sample purification, the removal of endotoxin may cause the loss of your sample. The loss rate is related to the binding strength of your target molecule and endotoxin, and the binding strength of your target molecule and endotoxin is related to the salt of the sample buffer. ion concentration, pH, etc. (2) In order to obtain a higher sample recovery rate and endotoxin removal rate, it is recommended that you store the samples to be processed at pH 7~8, salt. The total amount of endotoxin contained in the buffer solution with an ion concentration of 50mM~500mM is not more than 1/10 of the maximum load of the medium, and the purification process is explored according to the characteristics of your own samples. (3) The storage condition of this product is 20% ethanol, 4~8℃. |
